Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates
Identifieur interne : 004B63 ( Main/Exploration ); précédent : 004B62; suivant : 004B64Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates
Auteurs : Ying Zhu [République populaire de Chine] ; Mo Liu [République populaire de Chine] ; Weiguang Zhao [République populaire de Chine] ; Jianlin Zhang [République populaire de Chine] ; Xue Zhang [République populaire de Chine] ; Ke Wang [République populaire de Chine] ; Chunfang Gu [République populaire de Chine] ; Kailang Wu [République populaire de Chine] ; Yan Li [République populaire de Chine] ; Congyi Zheng [République populaire de Chine] ; Gengfu Xiao [République populaire de Chine] ; Huimin Yan [République populaire de Chine] ; Jiamin Zhang [République populaire de Chine] ; Deyin Guo [République populaire de Chine] ; Po Tien [République populaire de Chine] ; Jianguo Wu [République populaire de Chine]Source :
- Virus Genes [ 0920-8569 ] ; 2005-01-01.
Descripteurs français
- KwdFr :
- ADN complémentaire (), ADN complémentaire (isolement et purification), ARN viral (analyse), ARN viral (génétique), Adolescent, Analyse de séquence, Chine (épidémiologie), Données de séquences moléculaires, Génome viral, Humains, Microscopie électronique, Mutation, Mutation faux-sens, Mutation ponctuelle, Mâle, RT-PCR, Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Virulence (génétique), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Virus du SRAS (pathogénicité), Épidémiologie moléculaire, Évolution moléculaire.
- MESH :
- analyse : ARN viral.
- génétique : ARN viral, Virulence, Virus du SRAS.
- isolement et purification : ADN complémentaire, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Chine, Syndrome respiratoire aigu sévère.
- ADN complémentaire, Adolescent, Analyse de séquence, Données de séquences moléculaires, Génome viral, Humains, Microscopie électronique, Mutation, Mutation faux-sens, Mutation ponctuelle, Mâle, RT-PCR, Épidémiologie moléculaire, Évolution moléculaire.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Adolescent, China (epidemiology), DNA, Complementary (chemistry), DNA, Complementary (isolation & purification), Evolution, Molecular, Genome, Viral, Humans, Male, Microscopy, Electron, Molecular Epidemiology, Molecular Sequence Data, Mutation, Mutation, Missense, Point Mutation, RNA, Viral (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), SARS Virus (pathogenicity), SARS-CoV, Sequence Analysis, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Virulence (genetics), genomic analysis, isolation, mutation.
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : DNA, Complementary.
- geographic , epidemiology : China.
- chemical , genetics : RNA, Viral.
- chemical , isolation & purification : DNA, Complementary.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus, Virulence.
- isolation & purification : SARS Virus.
- pathogenicity : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Evolution, Molecular, Genome, Viral, Humans, Male, Microscopy, Electron, Molecular Epidemiology, Molecular Sequence Data, Mutation, Mutation, Missense, Point Mutation, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis.
Abstract
Abstract: Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. Hot spots of nucleotide variations and unique trends of mutations were identified on the viral genomes. Mutation rates were different from gene to gene and were correlated well with periodical or geographic characteristics of the epidemic.
Url:
- https://api.istex.fr/ark:/67375/VQC-RFQ7C39P-Z/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089183
DOI: 10.1007/s11262-004-4586-9
Affiliations:
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Le document en format XML
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Wuhan</orgName></affiliation></author><author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, 430072, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, 430072, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region 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Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, 430072, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name><affiliation wicri:level="4"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, 430072, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Genes</title><title level="j" type="abbrev">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Boston</pubPlace><date type="published" when="2005-01-01">2005-01-01</date><biblScope unit="volume">30</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="93">93</biblScope><biblScope unit="page" to="102">102</biblScope></imprint><idno type="ISSN">0920-8569</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-8569</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>China (epidemiology)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (isolation & purification)</term><term>Evolution, Molecular</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Microscopy, Electron</term><term>Molecular Epidemiology</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Mutation, Missense</term><term>Point Mutation</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (pathogenicity)</term><term>SARS-CoV</term><term>Sequence Analysis</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Virulence (genetics)</term><term>genomic analysis</term><term>isolation</term><term>mutation</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire ()</term><term>ADN complémentaire (isolement et purification)</term><term>ARN viral (analyse)</term><term>ARN viral (génétique)</term><term>Adolescent</term><term>Analyse de séquence</term><term>Chine (épidémiologie)</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Microscopie électronique</term><term>Mutation</term><term>Mutation faux-sens</term><term>Mutation ponctuelle</term><term>Mâle</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Virulence (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (pathogénicité)</term><term>Épidémiologie moléculaire</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Virulence</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Evolution, Molecular</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Microscopy, Electron</term><term>Molecular Epidemiology</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Mutation, Missense</term><term>Point Mutation</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Adolescent</term><term>Analyse de séquence</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Microscopie électronique</term><term>Mutation</term><term>Mutation faux-sens</term><term>Mutation ponctuelle</term><term>Mâle</term><term>RT-PCR</term><term>Épidémiologie moléculaire</term><term>Évolution moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. Hot spots of nucleotide variations and unique trends of mutations were identified on the viral genomes. Mutation rates were different from gene to gene and were correlated well with periodical or geographic characteristics of the epidemic.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name></region><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Liu, Mo" sort="Liu, Mo" uniqKey="Liu M" first="Mo" last="Liu">Mo Liu</name><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name><name sortKey="Zhang, Jianlin" sort="Zhang, Jianlin" uniqKey="Zhang J" first="Jianlin" last="Zhang">Jianlin Zhang</name><name sortKey="Zhang, Xue" sort="Zhang, Xue" uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name><name sortKey="Zhao, Weiguang" sort="Zhao, Weiguang" uniqKey="Zhao W" first="Weiguang" last="Zhao">Weiguang Zhao</name><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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